Extended Data Fig. 9: CBI1 blocks the mitochondrial translocation of BAX F116A.
a, b, Distribution of BAX F116A (a) or BAX F116A/C126A (b) (2 μM) between supernatant and BAX/BAK-deficient mitochondrial fractions, as detected by BAX western analysis after pre-treating BAX proteins with escalating doses of CBI1 (200 nM–4 μM, lanes 2–7), incubation with mitochondria, isolation of the supernatant and pellet fractions by centrifugation, and SDS PAGE. Isolation of the mitochondrial pellet fraction was verified by VDAC1 western analysis. The experiment was performed three times using independent preparations of mitochondria, proteins, and small molecule.
