Supplementary Figure 4: Histological characterization of E0771 tumors treated with AAV-p-MAEGI.

a-c, Representative pictures of H&E (a), CD8 (b), or Ki67 (c), staining of E0771 tumors collected from mice treated with PBS, AAV-Vector, or AAV-p-MAEGI. (Tumor harvest dpi = 36) (n = 2 out of 6 mice from two independent experiments with similar results). d, Quantification of % Ki67 positivity in E0771 tumors collected from mice treated with PBS, AAV-Vector, or AAV-p-MAEGI. Scale bars of zoomed-out images and insets = 200 μm. e-g, AAV infection efficiency assessed by intratumoral delivery of GFP-expressing AAVs and flow cytometry analysis. e, Percentage of GFP⁺ cells (mean ± s.e.m.) within tumors from mice 4 days after intratumoral injection of PBS (n = 3 mice) or AAV-GFP (n = 12), grouped by CD45⁺ and CD45^- cells. f, Percentage of GFP⁺ cells (mean ± s.e.m.) within tumors from mice 12 days after intratumoral injection of PBS (n = 2 mice) or AAV-GFP (n = 8), grouped by CD45⁺ and CD45^- cells. g, Percentage of GFP⁺ cells in various organs 4 days after intratumoral injection of AAV-GFP (n = 6 mice).