Extended Data Fig. 6: Evaluation of NK cells from WT and Irf4‒/‒ mice.
From: IRF4 expression by NK precursors predetermines exhaustion of NK cells during tumor metastasis
(a,b) Splenic NK cells from naïve WT and Irf4‒/‒ mice were stimulated with IL-12, IL-15, and IL-18. Shown are the percentages of IFN-γ+ and perforin+ cells (a), as well as granzyme B+ cells (b), within WT and Irf4‒/‒ NK cells. For IFN-γ, n = 5 mice per group; for perforin, n = 4 mice per group; for granzyme B, n = 3 mice per group; ns, not significant. (c) Splenic NK cells from naïve WT and Irf4‒/‒ mice were cocultured with RMA/S-GFP target cells at the indicated ratios. Percentage killing of target cells were illustrated. n = 3 biologically independent samples per group. (d) Percentages of granzyme B+ cells within lung NK cells, comparing WT versus Irf4–/– mice 20 days post B16-F10 cell intravenous injection. n = 3 mice per group; **P = 0.0038. (e) Expression of TIGIT in NK cells from the spleens of naïve WT and Irf4−/− mice. n = 4 mice per group; ns, not significant. (f) NK cells sorted from the spleens of naïve WT and Irf4−/− mice were stimulated in vitro with plate-bound anti-NK1.1 (PK136) antibody (20 µg/ml), IL-2 (10 ng/ml), and IL-15 (5 ng/ml) for 3 days, followed by assessment of TIGIT expression. n = 6 biologically independent samples per group; ****P < 0.0001. Data are presented as mean ± SD. Data were analyzed by a two-tailed unpaired Student’s t-test.
