Extended Data Fig. 7: Deleting IRF4 in Ncr1-expressing NK cells does not prolong survival in the melanoma metastasis model.
From: IRF4 expression by NK precursors predetermines exhaustion of NK cells during tumor metastasis
(a) Irf4fl/fl mice from Jackson Laboratory contain an eGFP gene positioned in the opposite orientation upstream of the Irf4 promoter. Upon Cre-mediated Irf4 deletion, eGFP is expressed, although at low levels in some cells. Shown is GFP expression in NK cells from the spleens of naïve Irf4fl/fl and Irf4fl/flNcr1Cre+/‒ mice. (b) Expression of Nkp46 in NK cells from the spleens of naïve Irf4fl/fl and Irf4fl/flNcr1Cre+/‒ mice. (c) Percentages of Nkp46+ cells among CD3‒NK1.1+ splenocytes in naïve Irf4fl/fl and Irf4fl/flNcr1Cre+/‒ mice. n = 3 mice per group; ns, not significant. (d) Percentage survival rates of Irf4fl/fl, Irf4w/wNcr1Cre+/‒, and Irf4fl/flNcr1Cre+/‒ mice following intravenous injection of B16-F10 cells. n = 5 mice per group; ns, not significant. Data are presented as mean ± SD (c). Data were analyzed by a two-tailed unpaired Student’s t-test (c) and a log-rank test (d).
