Extended Data Fig. 6: Complement constituents in human ChPs and AD plaques.
From: ApoE attenuates unresolvable inflammation by complex formation with activated C1q
a-b, Human ChP sections were stained for C1q (green) / C3 (red) (a) and C1q (green)/ApoE (red) (b). c,d, ChP sections were stained for CD68+ macrophages/DCs (c) and collagen IV (Col-IV) to mark basement membranes. Phase contrast shows lipid deposits in ChPs. e, ChP sections were stained for ApoE (green) and factor H (red); no primary antibody as control (NA). f,g, human brain sections were stained for Aβ (green) / ApoE (red) (left panels), Tau phosphorylation (pTau, green) / ApoE (red) (middle panels), and C1q (green) / ApoE (red) (right panels) (f). Blue for nuclei. No primary antibody as control (g). h, AD brain parenchyma sections were stained for C3 (red) / ApoE (green). Bar 100 μm for a-h; Data in a-h are representative images from at least 3 biologically independent samples.
