Extended Data Fig. 4: Quantification of 2D and 3D MSOT collagenmax signals in WT and DMD piglet muscles over time.
2D (a) and 3D (b) MSOT collagenmax signals of independent piglet muscles of surviving animals were compared with each other at weeks 1, 2, 3, and 4 of age. Each filled circle/square represents the mean ± s.d. MSOT signal of independent muscle regions over the course of the experiment (n = 12 WT/n = 8 DMD). 2D MSOT parameters were analyzed by post-hoc Tukey’s HSD following a mixed-effects models due to missing values in week 1 (litter 1). p values ≤ 0.05 were considered statistically significant. 3D MSOT collagen parameters were analyzed by Tukey’s honestly significant difference tests following a two-way (mixed design) ANOVA; Data are shown as mean ± s.d. n = 20 independent muscle regions (n = 12 WT/n = 8 DMD) in n = 5 biologically independent animals (n = 3 WT/n = 2 DMD piglets) from n = 2 litters are shown.
