Extended Data Fig. 3: Quantification of 2D and 3D MSOT collagenmax signals in WT and DMD muscles over time.
Quantification of 2D (a) and 3D (b) MSOT collagenmax signals in WT and DMD piglet muscles over time. WT and DMD MSOT collagenmax signals of independent piglet muscles of all animals were compared with each other at weeks 1, 2, 3, and 4 of age. Each filled circle represents one MSOT signal per independent muscle region (n = 24 WT/n = 20 DMD). Two-tailed independent samples t-tests (with Welch’s correction in cases of unequal variances) was used for statistical analysis. If the assumption of normal distribution was violated, a Mann-Whitney U-test was used. P values ≤ 0.05 were considered statistically significant. Bonferroni-Holm adjustment was used to control type I error, due to four comparisons (week 1 - 4) per parameter (e.g. 2D collagenmean). Confidence intervals (95% CI), effect size (R2), coefficients (t(df)/U) and exact p values are noted in the main text and/or Supplementary Tables. Data are shown as mean ± s.d. n = 44 independent muscle regions (n = 24 WT/n = 20 DMD) in n = 11 biologically independent animals (n = 6 WT/n = 5 DMD piglets) from n = 2 litters are shown.
