Extended Data Fig. 8: Cabozantinib does not induce apoptosis in Schwann cells or plexiform neurofibroma tumor tissues in vivo.
From: Cabozantinib for neurofibromatosis type 1–related plexiform neurofibromas: a phase 2 trial
a, Cabozantinib does not induce apoptosis by caspase 3/7 glo assay in human NF1-/- SC across of range of doses from 39 nM to 5 µM. By contrast, a robust increase in caspase 3/7 activity was observed in NF1-/- Schwann cells treated with Navitoclax (1 µM) as a positive control shown at the right. n = 3 independent cell culture wells were analyzed per condition over two independent experiments. ****Adjusted P-value < 0.0001 Navitoclax vs all other conditions (one-way ANOVA with Tukey’s multiple comparisons test). Data are presented as mean ± SD. b, Representative nerve tissues in cabozantinib (XL184) and vehicle treated mice are negative for TUNEL staining indicating that cabozantinb does not induce apoptosis. Positive and negative controls are shown at the top of the panel. The experiment was conducted two times independently with similar results.
