Extended Data Fig. 10: Evaluation of DeepGlioma on non-canonical diffuse gliomas.

A major advantage of DeepGlioma over conventional immunohistochemical laboratory techniques is that it is not reliant on specific antigens for effective molecular screening. a, A molecular oligodendroglioma with an IDH2 mutation is shown. DeepGlioma correctly predicted the presence of both an IDH mutation and 1p19q-codeletion. IDH1-R132H IHC performed on the imaged specimen is negative. The patient was younger than 55 and, therefore, required genetic sequencing in order to complete full molecular diagnostic testing using our current laboratory methods. b, A molecular astrocytoma with IDH1-R132S and ATRX mutations. DeepGlioma correctly identifies both mutations. c, A patient with a suspected adult-type diffuse glioma met inclusion criteria for the prospective clinical testing set. The patient was later diagnosed with a diffuse midline glioma, H3 K27-altered. DeepGlioma correctly predicted the patient to be IDH-wildtype without previous training on diffuse midline gliomas or other pediatric-type diffuse gliomas. We hypothesize that DeepGlioma can perform well on other glial neoplasms in a similar zero-shot fashions. Scalebar, 1 mm.