Extended Data Fig. 1: Quality control analysis of CRISPR- Cas9 screens in GBM models.
From: Targeting axonal guidance dependencies in glioblastoma with ROBO1 CAR T cells
(a) Gene-level LFC of core essential genes (CEGs) and non- essential genes across screens in pGBM (BT594) and rGBM (BT972) cells. All data presented from n = 3 biologically independent replicates; P values are from unpaired Student’s t-test; Median value at centre with lower and upper hinges at 1st (25th) and 3rd (75th) quartiles, respectively. Whiskers extend from hinge to 1.5*IQR. (b) Precision-recall plots of BT594 and BT972 screens. (c) Number of essential genes (FDR <0.05) identified in pGBM (BT594) and rGBM (BT972) cells. (d) Venn diagram of essential genes (FDR <0.05) in pGBM (BT594) and rGBM (BT972) cells after exclusion of CEGs. (e) Gene-level LFC of CEGs and non-essential genes from CRISPR screen in BT241 rGBM cells. All data presented as means from three biologically independent replicates. P values are from unpaired Student’s t-test. Median value at centre with lower and upper hinges at 1st (25th) and 3rd (75th) quartiles, respectively. Whiskers extend from hinge to 1.5*IQR. (f) Precision-recall plots of BT241 screen.
