Extended Data Fig. 3: Pan-PTP4A inhibition on rGBM invasion and JMS-053 permeability.
From: Targeting axonal guidance dependencies in glioblastoma with ROBO1 CAR T cells
(a) rGBM (BT972, MBT289, MBT298) invasive spheroid growth following treatment with DMSO (Control) or JMS-053 (4 µM or 8 µM). Data represented as mean ± s.e.; n = 3 experimental replicates. (b) Bi-directional Caco-2 permeability assessment of JMS-053 as compared to high (metoprolol) and low (nadolol and digoxin) permeability control compounds. Low permeability: Papp(A-B) ≤ 0.500 (x 106 cm/s); Moderate permeability: 0.500 <Papp(A-B) <2.50 (x 106 cm/s); High permeability: Papp(A-B) ≥ 2.50 (x 106 cm/s); average of 4 experimental replicates. (c) Parallel artificial membrane permeability assay (PAMPA) to evaluate blood brain barrier (BBB) permeability of JMS-053 as compared to high (propranolol) and low (atenolol) permeability controls. Asterisks indicate samples below the level of detection. Low permeability: Pe <1.00 nm/s; Moderate permeability: 1.00 <Pe <10.0 nm/s; High permeability: Pe > 10.0 nm/s.; average of 4 experimental replicates. ND, not determined.
