Fig. 4: Kinetics and magnitude of BM IgG ASC responses and of total and vaccine-specific serum IgG levels in the subject who donated three longitudinal BM aspirates over 2 years (subject 14).
a, ELISpot scanned images. The numbers of input ASC that were incubated were ~21K, ~40K and ~4.9K (2.5 months); ~14K, ~12K and ~3.8K (14 months); and ~58K, ~22K and ~7.2K (23 months) for PopA, PopB and PopD, respectively. b,c, The kinetics and magnitude of antigen-specific BM IgG ASC responses (b) and of total and antigen-specific IgG levels in the serum (c). Sera were collected within 1–5 months of the time of BM aspiration. Part of Fig. 4c is reproduced from Fig. 3a for the purpose of kinetics comparison. Sub, subject; K, 1,000. Counts were provided by the sorters. LLPC, boxes in a and b. All serum samples were tested at dilutions of 1:1,000–1:100,000 (total IgG) or 1:200–1:16,000 (antigen-specific IgG). For serum total and vaccine-specific IgG standard curves, see Supplementary Fig. 1. For details of subjects and samples, see Table 1.
