Extended Data Fig. 3: Exclusion of S2P BM IgA LLPC in SARS-CoV-2 mRNA vaccinees.
(a) Representative ELISpot scanned images. The numbers of input ASC that were incubated were ~9.6 K, ~3.7 K, and ~1.2 K (total) and ~58 K, ~22 K, and ~7.2 K (vaccine-specific) for PopA, PopB, and PopD, respectively. (b) Each symbol represents an individual vaccinee. Data were generated from 2, 3, and 3 different SARS-CoV-2 vaccinated subjects for PopA, PopB, and PopD, respectively. Statistics were assessed using Student’s t-test (two-tailed unpaired t-test) in Excel (Microsoft) and differences were considered significant at p values less than 0.05. ~: counts provided by the sorters; K: 1,000; LLPC: long-lived plasma cell (dotted boxes); Flu: influenza; Tet: tetanus. All ASC assayed at day 1 in culture. For individual ratios and statistic comparisons between any two antigens for any subset or between any two subsets for any antigen, see Supplementary Tables 3 and 4, respectively.
