Fig. 3: Histopathological characteristics of BRLs in mixed and active lesions.
a–c, BRLs (a), classical mixed lesions (b) and active lesions (c) stained for proteolipid protein (PLP) (brown) and HLA-DR (black). The dashed lines indicate the areas that were analyzed for subsequent quantifications. d–k, Comparison of densities of CD68+ (d), HLA-DR+ (e), IBA1+ (f), TSPO+ (g), iNOS+ (h), CD163+ (i), CD206+ (j) and TMEM119+ (k) cells in the rims of BRLs and of mixed and active lesion centers. l–o, Densities of CD3+ T cells (l), CD79a+ B cells (m), GFAP+ astrocytes (n) and TPPP/p25+ oligodendrocytes (o). p, BRLs had a higher proportion of iron rims compared to classical mixed lesions. q–aa, Comparison of myeloid cell features, lymphocyte infiltration and oligodendrocyte numbers in BRLs with and without iron rims and in mixed lesions without iron. CD68+ (q), HLA-DR+ (r), IBA1+ (s), TSPO+ (t), iNOS+ (u), CD163+ (v), CD206+ (w) and TMEM119+ (x) cells, and CD3+ T cells (y), CD79a+ B cells (z) and TPPP/p25+ oligodendrocytes (aa). d–o,q,aa, In the violin plots, P values were determined, depending on normality, using a Brown–Forsythe or a one-way Welch’s ANOVA and Dunnett’s test for multiple comparisons (d,f,k,o,r–u,aa) or Kruskal–Wallis test (one-way ANOVA) and Dunn’s multiple comparison test (e,g–j,l–n,q,v–z). p, The P value was determined using a Fisher’s exact test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P < 0.0001.
