Extended Data Fig. 10: Knocking out astrocytic Panx1 shows protective effect for ischemic stroke in mice.
a. The consequence of Inflare blockage by astrocytic Panx1 knockout on MCAO outcome. The AAV-PHP. eB with astrocytic expressing of mCherry or cre was orbitally injected into the Panx1flox/flox mice for whole brain astrocytic Panx1 knockout. b. The brain infarction in Sham, flox mice with MCAO (M-flox) or astrocytic Panx1 knockout mice with MCAO (M-cKO) at 3 days after surgery, p = 0.027 between M-flox and M-cKO. c. The neurological deficient scores in different groups at 3 days after surgery (n = 3 mice for Sham; n = 4 mice for M-flox; n = 5 mice for M-cKO). d. The trajectory of mice (left) and total movement distances (right) in the open field test over 5 minutes (n = 3 mice for Sham; n = 4 mice for M-flox; n = 5 mice for M-cKO). e. The total time that mice maintained on the balance beam (n = 3 mice for Sham; n = 4 mice for M-flox; n = 5 mice for M-cKO). Scale bars, 50 µm. Data are shown as the mean ± s.e.m. *, p < 0.05; All statistical tests are two-sided and see Supplementary Table 1 for statistics.
