Fig. 7: Differential intercellular signaling capacity identified in the ME and cortex. | Nature Neuroscience

Fig. 7: Differential intercellular signaling capacity identified in the ME and cortex.

From: Characteristics of blood–brain barrier heterogeneity between brain regions revealed by profiling vascular and perivascular cells

Fig. 7

a, Alluvial plot showing the number of significant (P < 0.05) co-expressed EC ligands and perivascular receptors. b, Co-immunostaining for BSG (white) and its receptor integrin α6 (ITGA6, red) in cortex and ME, validating elevated expression of ligand (BSG) and receptor (ITGA6) in cortex. c, Co-immunostaining for CD31 (white), AQP4 (green) and ITGA6 (red) in cortex. d, Co-immunostaining for VEGFR2 (red), EMCN (cyan, ME ECs) and GLUT1 (green, cortex ECs) in cortex and ME. EMCN and GLUT1 were used to label ECs instead of CD31 owing to antibody compatibility with VEGFR2. e, Co-immunostaining for VEGF (white), EMCN (cyan, ME ECs) and (GLUT1, green, cortex ECs) in cortex and ME. EMCN and GLUT1 were used to label ECs instead of CD31 owing to antibody compatibility with VEGF. f, Immunostaining illustrating complementary spatial distribution of ligand VEGF (white) and receptor VEGFR2 (red) in ME. Non-ME vessels are labeled in green (GLUT1).

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