Extended Data Fig. 2: Other fiber photometry results for DA release in the NAc and mPFC.
a,b, Schematics of viral expression and optic fiber placement in the NAc shell (a) and core (b) for fiber photometry. c–g, Representative image of viral expression in the NAc shell (c) and core (e). Scale bar = 0.5 mm. DA release in the NAc shell (d) and core (f) and AUC comparison (g) after METH (0.1 mg kg−1) infusion in mice (shell: sub, n = 9; dom: n = 10; core: sub, n = 7; dom, n = 6). Two-way ANOVA followed by Sidak’s post-hoc test, *P < 0.05, **P < 0.01. h–k, Schematics for viral expression and optic fiber placement in the NAc (h) and mPFC (j) for fiber photometry experiments. Saline-induced DA release in the NAc (i) and mPFC (k) in mice (NAc: n = 13/group; mPFC: n = 9/group). Two-sided unpaired t test. l, Timeline and schematic showing the METH self-administration with simultaneous fiber photometry recording. m,n, Schematics showing viral expression and optic fiber placement in the NAc (m) and mPFC (n) for fiber photometry in a free-moving self-administration. o,p, DA release in the NAc (o) and mPFC (p) following active and inactive pokes in a free-moving METH self-administration (NAc: n = 10 per group; mPFC: sub, n = 8; dom, n = 9). Two-sided unpaired t test, *P < 0.05, **P < 0.01. Each row in the heatmap represents averaged DA dynamics over trials from individual mouse (ranked by AUC). Data are represented as mean ± s.e.m. Detailed statistical information is available in Supplementary Table 1.
