Extended Data Fig. 5: Endogenous N-myc condensates contain DNA-binding and dimerization partner, transcriptional machinery and nascent RNA but do not co-localize with c-Jun.
From: MYC phase separation selectively modulates the transcriptome
Immunostained N-myc condensates colocalized with MED1 (a), Pol II S5p (b), MAX (c), nascent RNA (d) and c-Jun (e). Kelly cells were stained with antibody against N-myc, and antibodies against MED1, Pol II S5p, or c-Jun, or co-expressed with MAX-mKO3, or labeled by 5-ethynyluridine for labeling nascent RNA. The arrows point to representative condensates. The fluorescence intensity profile (right panel) is extracted from the position shown by the dashed line. Co-localization assessment by Pearson’s correlation coefficient is shown in right panels. Center lines show the median values. Green boxes contain the 25th to 75th percentiles of dataset. Black whiskers mark the 10th and 90th percentiles. Outliers are marked with grey dots. NMED1 = 76, NPol II-S5P = 67, NMAX = 61, Nnascent RNA = 82 cells, Nc-Jun = 67 cells. Scale bars, 5 μm.
