Extended Data Fig. 9: Saturation concentration of SparkDrop-tagged N-myc and the stable N-myc/SparkDrop SH-EP cells used for the RNAseq experiments.
From: MYC phase separation selectively modulates the transcriptome
(a) Saturation concentration of SparkDrop-tagged N-myc with and without lenalidomide. Each green dot represents data from a single cell (~200 cells for each group). The concentration was calculated based on the green fluorescence of mEGFP in SparkDrop. (b) Representative images of stable SparkDrop-MYCN cells before and 30 minutes after 1 µM lenalidomide (lena) incubation. Scale bar: 20 µm. (c) N-myc concentration is 0.20 ± 0.01 µM (Mean ± SD). N = 200 cells.
