Extended Data Fig. 8: Structural conservation of the yeast ceramide synthase.

A Cartoon representation of Lac1 color-coded by sequence-conservation with data from Supplementary Fig. 10a. B Cartoon representation of Lag1 color-coded by sequence-conservation with data from Supplementary Fig. 11. Yellow areas denote regions of no conservation. The catalytic residues are highlighted as spheres. C Luminal view of the cartoon-representation of superimposed Lag1 (yellow) and Lag1 (grey), color-coded by Cα root-mean-square deviation (r.s.m.d.). Catalytic residues and C26:0 are shown as sticks. D Pair-based sequence alignment of Lac1 Saccharomyces cerevisiae (P28496) and Lag1 Saccharomyces cerevisiae (P38703). Sequences are color coded by conservation with a cut-off at 30 %. Catalytic residues are highlighted in red. Non-resolvable residues from cryo-EM are marked in light gray. Alignment was prepared with Jalview. E Comparison of local densities in Lag1 and Lac1 for density-based subunit differentiation. The corresponding sequences are highlighted in yellow in the alignment (D).