Fig. 2

Technical validation of the transcriptomic data. (A) Overview of the RNA-seq data. Raw reads (top panel), raw data information (second panel), Q30 (%) (third panel) and GC (%) (bottom panel) for each RNA-seq library were plotted. (B) A heatmap showing the correlation coefficients of the scaled expression levels among all sample pairs. R2 and R4 are two wildtype strains of S. fredii with different host compatibilities. R2p4, R2 with NopP from R4 swapped in; R2i4, R2 with NopI from R4 swapped in; R4p2, R4 with NopP from R2 swapped in; R4i2, R4 with NopI from R2 swapped in; rhcN, R2 T3SS mutant; Unino, uninoculated; Nod, nodule; r1, r2 and r3, replicates 1, 2 and 3.