Figure 2

(A): Observation of wound-site macrophages. (a): Immunostaining on E13-24 h. Green (F4/80), red (CD31), and blue (4′,6-diamidino-2-phenylindole [DAPI]) staining. Scale bar = 100 µm. (b): Enlarged image showing the F4/80-positive linear structure. Yellow arrows (wound ridge) and white arrows (tunneling nanotube [TNT]). Scale bar = 100 µm. (B): Macrophage-phagocytic enlarged images at the wound center. This image shows F4/80-positive macrophages engulfing multiple cells. Scale bar = 50 μm. (C): Macrophage phagocytosis after toluidine blue staining on E13-24 h. White arrows indicate phagocytic cells. Scale bar = 100 µm. (D): Overview of the images at each time point. The number of phagocytic cells per 10 slices (1 μm/slice) around the wound center is measured. Scale bar = 100 µm.