Figure 7
Validation of senescence-related model from a single-cell perspective. (a) Cell annotation of scRNA-seq from GSE132465. (b) AUC scoring of senescence-related model genes. The threshold was determined as 0.054 and the 39,823 cells exceeded the threshold value. (c) The t-SNE plots based on the AUC score of cells. Cell subsets with high AUC score are highlighted. (d) Top-correlated ligand activities inferred in epithelial cells (tumor cells) according to NicheNet. (e) Dot plots showing the intensity (dot intensity) and the expression percentage (dot size) of top- correlated ligands in epithelial cells (tumor cells). (f) Ligand-receptor pairs displaying potential interactions between epithelial cells (tumor cells) and surrounding cells. (g) Heatmap showing regulatory potential of top-correlated ranked ligands in epithelial cells (tumor cells) and the downstream target genes in surrounding cells.
