Fig. 2

Subcellular localization of CISD3 in pan-cancer. (A) Heatmap of CISD3 expression in single-cell subpopulations across pan-cancer. UMAP plots showing cell type distribution annotations and CISD3 expression analysis in NSCLC (B), CRC (C), and KIRC (D). Spatial transcriptomic deconvolution maps illustrating cell localization in LUSC (E), CRC (F), and KIRC (G). Each dot represents a spot in spatial transcriptomic sequencing; the darker the color (red), the higher the content of that cell type in the spot. Spearman correlation of CISD3 expression with microenvironment components at spatial transcriptomic resolution in LUSC (H), COAD (I), and KIRC (J). Red lines indicate positive correlations, green lines indicate negative correlations, gray lines indicate non-significant correlations, and the thickness of the lines represents the absolute value of the correlation coefficient. In the triangular areas, red squares represent positive correlations, blue squares represent negative correlations; the more significant the p-value, the darker the color, the larger the absolute value of the correlation coefficient, and the larger the square.