Fig. 3 | Scientific Reports

Fig. 3

From: Proton pump inhibitors reduce chemotherapeutic hepatotoxicity and enhance hepatic uptake and accumulation of drug-loaded extracellular vesicles

Fig. 3

PPIs promote RBC-EVs/Trim21-ASO-mediated inhibition of macrophage inflammatory responses. (A–C) The morphology (A), size distribution (B) and EV markers (C) of the RBC-EVs were evaluated by transmission electron microscopy. Scale bar, 500 nm (A), nanoparticle tracking analysis (B) and western blot (C), respectively. (D) Cy3-conjugated cholesterol-modified Trim21-ASOs were loaded into RBC-EVs and analyzed by flow cytometry. (E) PEMs were treated with 10 µg RBC-EVs/Trim21-ASOs for 48 h. TRIM21 protein expression in PEMs was detected by western blot. (F) PEMs were cocultured with 10 µg RBC-EVs/Trim21-ASOs for 48 h together with or without 10 µM Rabe and then stimulated with 50 µM PA for 12 h. IL-1β, IL-6 and TNF secreted from these cells were measured by ELISA. *P < 0.05; **P < 0.01 (one-way ANOVA followed by Tukey test; mean ± SD).

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