Fig. 4

Investigation of fat discrimination via CC-DOP imaging of human breast tissues. White-light images (rows A) and stained histology slices (rows B) are shown for two healthy specimens (H&E-stained) and two cancer specimens (ORO-stained). Fat/non-fat segmentation masks (rows C) indicate the locations of the fat and non-fat regions of the specimens, which are superimposed on the LL-DOP images (row D) and on the CC-DOP images (row F) to enable the fat/non-fat histograms shown respectively in row E and row G. Row H shows ROC curves with corresponding AUC values to evaluate fat segmentation performance of CC-DOP images, indicating strong fat discrimination for each specimen with exception of Healthy Specimen 2 which shows some fat discrimination. Overall, CC-DOP values appear to correlate with fat content (higher values) and potentially dense-cellular content (high magnitude negative values).