Fig. 6
From: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation
Hepatocyte-derived ANGPTL4 is crucial for suppressing inflammatory activation in KCs. (A) Schematic of the experimental procedure: co-culture of HCs with LPS-stimulated KCs. (B) ELISA analysis of ANGPTL4 levels in the KCs culture medium. (C) RT-qPCR and ELISA analysis of IL-10, IL-1β, and TNF-α expression levels. (D) WB analysis of polarization-related proteins in KCs, with quantitative assessment based on band intensity. (E) Flow cytometry analysis of M1-polarized cells, identifying KCs with FITC-labeled CD68 and M1 markers with APC-labeled CD86. (F) Western blot analysis of proteins related to the NF-κB pathway, with quantification based on band intensity. (G) Immunofluorescence staining to localize phosphorylated p65. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.
