Fig. 7
From: ANGPTL4 induces Kupffer cell M2 polarization to mitigate acute rejection in liver transplantation
Knockdown of ANGPTL4 expression in HCs within the co-culture system promotes M1 polarization of KCs. (A) Schematic representation of the experimental procedure: siRNA-mediated knockdown of ANGPTL4 in HCs co-cultured with LPS-stimulated KCs. (B) RT-qPCR and ELISA assessments of IL-10, IL-1β, and TNF-α levels. (C) WB analysis of polarization-related proteins in KCs, with quantification of band intensity. (D) Flow cytometry of M1-type polarized cells, with FITC-labeled CD68 and APC-labeled CD86. (E) Western blot assessment of proteins associated with the NF-κB pathway, including band intensity quantification. (F) Immunofluorescence staining to identify the localization of phosphorylated p65. ns P > 0.05, *P < 0.05; **P < 0.01; ***P < 0.001; n = 3.
