Fig. 4

CD206⁺ M2 macrophage-derived Tgf-β1 gene deletion enhanced APs proliferation. (A) Relative mRNA expression analysis of cell cycle-related genes in SVF from eWAT (n=6; n=6). (B) Schematic for magnetic activated cell sorting Pdfgrα⁺ cells from SVF of eWAT. (C) Relative mRNA expression analysis of cell cycle-related genes in PDGFRα⁺ cells from eWAT (n=3; n=3). (D) Representative confocal images indicate PDGFRα co-localization with mKi67 (scale bar = 20 μm, n=4; n=4). (E) Quantification of PDGFRα⁺ mKi-67⁺ DAPl⁺ (n=4; n=4). Data represent mean ± SEM. Statistical analysis was performed using a two-tail Student’s t-test (*p < 0.05, **p<0.01). PDGFRα, Platelet-derived growth factor receptor alpha.