Fig. 8 | Scientific Reports

Fig. 8

From: Deletion of IRS-1 leads to growth failure and insulin resistance with downregulation of liver and muscle insulin signaling in rats

Fig. 8

The insulin-stimulated phosphorylation of Akt, GSK3β, and S6K in the liver and skeletal muscle of WT and IRS-1 KO rats. Eight-week-old male WT and KO rats were fasted overnight, and insulin or vehicle was subsequently injected into the inferior vena cava. The liver and gastrocnemius muscle were excised at 15 min after the insulin injection. (A–E) Insulin-stimulated phosphorylation of Akt, GSK3β, and S6K in the liver. (A) Representative immunoblots. (B–E) The immunoreactivities of Thr308 phosphorylation of Akt (B), Ser473 phosphorylation of Akt (C), Ser9 phosphorylation of GSK3β (D), and Thr389 phosphorylation of S6K (E) were quantified and divided by the immunoreactivity of β-tubulin. (F–J) Insulin-stimulated phosphorylations of Akt, GSK3β, and S6K in the gastrocnemius muscle. (F) Representative immunoblots. (G–J) The immunoreactivities of Thr308 phosphorylation of Akt (G), Ser473 phosphorylation of Akt (H), Ser9 phosphorylation of GSK3β (I), and Thr389 phosphorylation of S6K (J) were quantified and divided by the immunoreactivity of β-tubulin. Values are means ± SDs (n = 7). The two-way ANOVA results are shown below the graph (*p < 0.05, NS; not significant). The results of Tukey-Kramer post-hoc test are shown in the graph when there was a significant interaction of genotype and insulin stimulation (†p < 0.05).

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