Fig. 4
CITRUS analysis of flow cytometry data revealed non-T cell apheresis-dependent differences. Flow cytometry data were fully recompensated and uploaded into Cytobank. CITRUS analysis was performed using PAMR as predictive model. Absolute numbers of events were determined in the three non-T cell metaclusters (a) # 379956, (b) # 379974 and (c) # 379962. (n = 15 for Trima 6, n = 9 for Trima 7 and n = 14 for Trima 7 PAS; statistical analyses were carried out using one-way ANOVA with Tukey test for multiple comparisons; effect size is reported as Cohen’s d). Heatmaps were generated displaying MFI per channel to phenotypically characterize the selected clusters (one representative sample per condition is shown as an example).
