Fig. 1
From: Unraveling chain specific ubiquitination in cells using tandem ubiquitin binding entities
Graphical representation of ubiquitin chain-specific TUBE based assay platform. Cellular signaling or exogenous ligands such as PROTACs and MGs can induce ubiquitination of target proteins that are diverse in linkage configuration. Functionally, monoubiquitination and multimonoubiquitination are important for protein trafficking, K48 and K11 chains are associated with proteasomal degradation and K63-chains are involved in autophagy, inflammation and DNA repair and Linear chains mediate NF-κB activation. Polyubiquitin binding TUBEs facilitate selective capture and detection of context-dependent polyubiquitination of endogenous proteins in a high throughput manner.
