Fig. 3 | Scientific Reports

Fig. 3

From: Surface display of Nocardia seriolae HRP1 on Bacillus subtilis and its application as live vaccine for largemouth bass

Fig. 3

Successful construction of HRP1-cotC-pDG364 recombinant plasmid and identification of CotC-HRP1 fused protein of recombinant spores by Western blotting. (A) Schematic diagram of HRP1-cotC-pDG364 recombinant plasmid construction. (B) Confirmation of positive clones by PCR after recombinant plasmid transformation. Lane M: DNA marker, Lane 1–2: randomly selected negative clones, Lane 3–4: randomly selected positive clones. (C) The 3D structure of the HRP1 antigen protein and the ___location of the three main epitopes site. (D) The expression of cotC-HRP1fusion protein of recombinant spores by Western blotting. Lane M: protein marker, Lane 1: protein of B. subtilis spores, Lane 2: protein of recombinant HRP1-cotC-B. subtilis spores, Lane 3: negative control.

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