Fig. 4

MYH9 promotes epithelial-mesenchymal transition signaling. (A,B) MYH9 protein level was measured by western blot in U87 and U251 cells transfected with siMYH9. GAPDH was used as a loading control. (C–E) MYH9 knockdown reduced cell migration and invasion identified using Transwell and Boyden assays in U87 and U251 cells. (F–H) MYH9 knockdown reduced the expression of β-catenin and EMT signals in U87 and U251 cells. Data are presented as the mean ± SD for three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.