Fig. 3

Overexpression of DRAM1 promoted the migration, proliferation, and metastasis of gastric cancer cells. (a) The effect of DRAM1 on cell migration was evaluated using wound-healing assay, and the results showed that DRAM1 overexpression markedly promoted the migration of gastric cancer cell. (b) Bar chart showed the difference in migration area between DRAM1 overexpressing GC cells and NC cells (NC vs OE, **: P = 0.002). (c) Plate cloning experiments confirmed that DRAM1 overexpression promoted the proliferation of gastric cancer cells. (d) Bar chart showed the number of DRAM1 overexpressing GC cells and NC cells analyzed by plate cloning experiments (NC vs OE, ***: P < 0.001). (e) The Transwell assay confirmed that overexpressing DRAM1 promoted gastric cancer cell migration and metastasis. (f) CCK8 assay confirmed that overexpressing DRAM1 promoted the proliferation of gastric cancer cells (NC vs OE, ****: P < 0.001). (g) EDU assay confirmed that knockdown of overexpressing DRAM1 promoted the proliferation of gastric cancer cells. Scale bar = 100 μm. (h) Western blotting was used to detect EMT pathway-related proteins between DRAM1 overexpressing GC cells and NC cells. Compared with NC gastric cancer cells, the expression of N-cadherin in gastric cancer cells with DRAM1 overexpression was up-regulated, and the expression of E-cadherin was down-regulated.