Fig. 4

Silencing DRAM1 promoted apoptosis of gastric cancer cells, while overexpression of DRAM1 inhibits apoptosis of gastric cancer cells. (a) The apoptosis of DRAM1 knocked down gastric cancer cells and NC gastric cancer cells was detected by flow cytometry. (b) Bar chart showed that knockdown of DRAM1 promoted apoptosis in gastric cancer cells (Ctrl vs shDRAM1#1, ***: P < 0.001; Ctrl vs shDRAM1#2, ***: P < 0.001). (c) The apoptosis of DRAM1 overexpressing gastric cancer cells and NC gastric cancer cells was detected by flow cytometry. (d) Bar chart showed that DRAM1 overexpressing inhibited apoptosis in gastric cancer cells (NC vs OE, *: P = 0.013). (e) Western blotting was used to detect apoptotic proteins in DRAM1 knocking gastric cancer cells and NC gastric cancer cells. (f) Protein quantitative analysis showed that knockdown of DRAM1 up-regulated the expression of Bax (Ctrl vs shDRAM1#1, *: P < 0.015; Ctrl vs shDRAM1#2, *: P < 0.031)and down-regulated the expression of Bcl-2 (Ctrl vs shDRAM1#1, ***: P < 0.001; Ctrl vs shDRAM1#2, ****: P < 0.001). (g) Detecting apoptotic proteins in DRAM1-overexpressing gastric cancer cells and NC gastric cancer cells by Western blotting. (h) Bar chart showed that overexpressed DRAM1 up-regulated Bcl-2 (NC vs OE, ***: P < 0.001) expression and down-regulated Bax (NC vs OE, ****: P < 0.001) by Western blotting analysis.