Fig. 7 | Scientific Reports

Fig. 7

From: DRAM1 enhances the proliferation and metastasis of gastric cancer through the PI3K/AKT/mTOR signaling pathway and energy metabolism

Fig. 7

Knockdown of DRAM1 inhibited the growth of subcutaneous tumors in nude mice. (a) Knocking down of DRAM1 inhibited subcutaneous growth in nude mice through vivo experiments (n = 3). (b) Knockdown of DRAM1 significantly inhibited tumor size in nude mice (n = 3). (c) Knockdown of DRAM1 significantly inhibits the volume of tumors in nude mice by measuring mouse volume (Ctrl vs shDRAM1#1, **: P = 0.002). (d) Knocking down DRAM1 remarkablely inhibited tumor weight in nude mice (Ctrl vs shDRAM1#1, ****: P < 0.001). (e) Western blot analysis of nude mouse tumor tissues showed that knockdown of DRAM1 significantly inhibited the expression of p-AKT and p-mTOR. (f) HE staining of DRAM1 knockdown tumor tissues and control tissues. Scale bar = 100 μm. (g) DRAM1 expression in tumor tissues and control tissues with DRAM1 knockdown was detected by IF. Scale bar = 100 μm. (h) Immunofluorescence assay confirmed that knockdown of DRAM1 significantly inhibited the expression of ki-67 in tumor tissues. Scale bar = 100 μm. (i) Tunel staining confirmed that knockdown of DRAM1 promotes apoptosis in nude mice. Scale bar = 100 μm.

Back to article page