Fig. 1 | Scientific Reports

Fig. 1

From: Mutation of the gidB gene causes intrinsic streptomycin resistance in Bacillus velezensis

Fig. 1

Method for exchanging the thymine at the 58th nucleotide position of the gidB gene of B. velezensis DMB07 into cytosine using pIMAY. (A) Schematic of allelic exchange using pIMAY via splicing overlap extension-PCR. (B) PCR profiles of DMB07gidB. The names and locations of the primers are shown in panel A. Lanes: M, molecular size markers (NICSROgene 100 bp DNA Ladder, BIONICS, Seoul, Korea); 1, strain DMB07; 2, DMB07::pIMAY-tgidBT58C; 3, DMB07gidBT58C.

Back to article page