Table 4 Reproducibility of the Real-Time qPCR assay (n = 3).
From: Establishment and validation of a simple and accurate qPCR detection method for Haemophilus parasuis
Concentrations of standard plasmid | Intra-batch variance | Inter-batch variance | ||
|---|---|---|---|---|
Mean ± SD | CV (%) | Mean ± SD | CV (%) | |
3.16 × 106 copies/µl | 20.11 ± 0.04 | 0.20 | 20.96 ± 0.16 | 0.76 |
3.16 × 105 copies/µl | 23.44 ± 0.08 | 0.34 | 24.40 ± 0.15 | 0.61 |
3.16 × 104 copies/µl | 27.05 ± 0.09 | 0.33 | 27.74 ± 0.21 | 0.76 |
