Fig. 13

Nrf2 inhibitor ML385 reversed the enhancing effect of remimazolam on the mitochondrial membrane potential (MMP). (A) The MMP of cells was measured using a JC-1 assay. The representative images of JC-1 aggregates and JC-1 monomers reflexed the MMP of primary hippocampal neurons. The scale bar represents 200 nm. (B) The ratio of JC-1 aggregates to JC-1 monomers was decreased in neurons administrated with ML385 prior to remimazolam, suggesting that ML385 inhibited the elevation of MMP by remimazolam. Results are shown as means ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001.