Table 3 Primer sequences used in qRT-PCR.
From: The inhibitory effect of Osthole on A549 lung adenocarcinoma cells and its biomarker
Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
IL-1β | ATGATGGCTTATTACAGTGGCA | AAGCCCTTGCTGTAGTGGTG |
EGF | GTCTTGACTCTACTCCACCCC | AGCCAACAACACAGTTGCAT |
IL-2 | TCACAGTAACCTCAACTCCTGC | GTTTCAGTTCTGTGGCCTTCTT |
IL-6 | TTCGGTCCAGTTGCCTTCTC | TCACCAGGCAAGTCTCCTCA |
IL-10 | CACCTCCGCCAATCTCTCAC | GATGCCTTTCTCTTGGAGCTTA |
TNF-α | TAGCCCATGTTGTAGCAAACC | GCTCTTGATGGCAGAGAGGA |
TGF-β1 | CTGGCGATACCTCAGCAACC | GCCGGTAGTGAACCCGTTGAT |
Smad | GGAGGTGGCCTGATCTTCAC | CTTGGTGGATGCTGGATGGT |
E-cadherin | GCTGGACCGAGAGAGTTTCC | CAAAATCCAAGCCCGTGGTG |
CK18 | ACTGGTCTCAGCAGATTGAGG | TTCTCCAAGCTGGCCTTCAG |
ZO-1 | CGTAGTAGGTCGGGTTTTCTTT | CATTGCTGTGCTCTTACTGTGG |
AQP5 | CGCTCAACAACAACACAACG | CAGTCCTCGTCAGGCTCAT |
N-cadherin | TGAAAGACCCATCCACGCC | CACGAAGCCAAGGTCATAGGT |
Snail | GGTTCTTCTGCGCTACTGCT | TGCTGGAAGGTAAACTCTGGAT |
ZEB | AGCGTCAAATAGTGTGTGTTCCA | TTGCCCTTCCTTTCCTGTGTC |
Twist | CAGAGCGACGAGATGGACAA | CGGACAGCCCTGGCG |
PGC-1α | CTACGGCTCCTCCTGGGAAA | CAACCAGAGCAGCACAAAAGT |
Vimentin | AAGCTGCAAGCCAAGGAAGC | ACAACCCACCACGTAGACAG |
α-SMA | TTCCATCGTCCACCGCAAAT | AAGAAAGTGACTGCGGGGTG |
Slug | TAGCGAGTGGTTCTTCTGCG | AGGGCTGCTGGAAGGTAAAC |
STAT1 | GTTCGGCAGCAGCTTAAAAAGT | CTCATTCACATCTCTCAACTTCAC |
STAT3 | CTCCTTGCCAGTTGTGGTGA | GTTCCAATTGGGGGCTTGGT |
SIRT1 | GCGGTTCCTACTGCGCGA | CACCGAACAGAAGGTTATCTGG |
TFAM | TTATAGGGCGGAGTGGCAGG | TAAGCTGAACGAGGTCTTTTTGG |
NRF-1 | ACCTTGAGTACAGGGACAGC | TTTTGAGCAAGACGTGGAGAA |
β-actin | CACGCAGTACAGCAAGGTCC | CACGCAGTACAGCAAGGTCC |
