Fig. 4 | Scientific Reports

Fig. 4

From: Astragalus mongholicus and Hedyotis diffusa willd inhibit cell proliferation by attenuating the miR-582-3p-p27 signaling pathway in LUAD

Fig. 4

p27 is a direct target of miR-582-3p. (A) The expression of p27 decreased in LUAD tissues compared to paracancerous tissues, Wilcoxon rank sum test, 95% CI: −0.317–0.070, (J-I): −0.188. (B, C) The expression of p27 decreased in lung cancer cells compared to 16HBE. (D) The predicted wild-type or mutated miR-582-3p binding sites in the 3’-UTR of p27 mRNA. (E) The luciferase report assay demonstrated that the overexpression of miR-582-3p could reduce the intensity of fluorescence in A549 cells transfected with the p27-WT vector, while it did not affect the p27-MUT vector. (F) RNA pull-down experiment demonstrated miR-582-3p can directly interact with p27. (G) The expression of p27 was negatively correlated with miR-582-3p from TCGA, Spearman’s Rank Correlation Coefficient Test. r=−0.088. (H-J) The expression of p27 in LV-miR-582-3p mimic or LV-miR-582-3p inhibitor A549 cells was measured by WB and RT-qPCR. Data were presented as mean ± SD for three individual experiments. one-way ANOVA, *P < 0.05, **P < 0.01.

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