Fig. 5 | Scientific Reports

Fig. 5

From: Astragalus mongholicus and Hedyotis diffusa willd inhibit cell proliferation by attenuating the miR-582-3p-p27 signaling pathway in LUAD

Fig. 5

miR-582-3p knockdown inhibited the proliferation and arrested cell cycle at the G0/G1 phase in A549 cells by upregulating p27 expression. (A) shRNA-p27#2 construct exhibited the best interference effects. (B, C, E) The CCK-8 and colony formation assays demonstrated that the inhibition of miR-582-3p resulted in reduced proliferation through the upregulation of p27 and flow cytometry analysis revealed that the inhibition of miR-582-3p resulted in the suppression of cell cycle progression through the upregulation of p27 (D, F). Data were presented as mean ± SD for three individual experiments, one-way ANOVA, **P < 0.01.

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