Fig. 1: Methionine deprivation exerts inhibitory effects on glioma in vitro and in vivo. | npj Precision Oncology

Fig. 1: Methionine deprivation exerts inhibitory effects on glioma in vitro and in vivo.

From: Methionine deprivation inhibits glioma proliferation and EMT via the TP53TG1/miR-96-5p/STK17B ceRNA pathway

Fig. 1

A Cell viability of glioma cells is assessed using the MTT assay after culturing with methionine-deprived medium for 48 h. B The size and number of colonies are observed using colony formation experiments after culturing glioma cells in methionine-deprived medium. C EdU assay is used to examine cell proliferation after culturing glioma cells in methionine-deprived medium for 48 h. The scale bar is 100 μm. D Transwell assay is used to assess the migration and invasion ability after culturing LN229 and U251 cells in methionine-deprived medium. The scale bar is 100 μm. E Tumor volume and weight of normal or methionine deprivation feeding group nude mice. Error bars represent mean ± standard deviation (SD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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