Fig. 2: Methionine deprivation affects the lncRNA TP53TG1/miR-96-5p/STK17B ceRNA network. | npj Precision Oncology

Fig. 2: Methionine deprivation affects the lncRNA TP53TG1/miR-96-5p/STK17B ceRNA network.

From: Methionine deprivation inhibits glioma proliferation and EMT via the TP53TG1/miR-96-5p/STK17B ceRNA pathway

Fig. 2

A Two intersecting genes (TP53TG1 and LINC00461) are obtained. B Kaplan–Meier survival curves are generated in patients with high and low TP53TG1 or LINC00461 expression in glioblastoma multiforme and low-grade gliomas obtained from the TCGA databases. C A network diagram representing associations was constructed. D, E The differential expression and correlation analyses of the 68 mRNAs. F The protein levels of STK17B in normal and high-grade glioma patient tissues. G GSEA reveals high TP53TG1 and STK17B expression levels associated with epithelial mesenchymal transition (EMT). H qRT-PCR employed to assess the relative expression levels of TP53TG1 and miR-96-5p after 48 h of methionine deprivation. I The STK17B protein level after 48 h of methionine deprivation in LN229 and U251 cells. Error bars represent mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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