Fig. 2: Single-cell analysis of T cell subsets. | npj Precision Oncology

Fig. 2: Single-cell analysis of T cell subsets.

From: G0S2: a potential target for NSCLC identified through prognostic models from multi-Omic analysis of regulatory T cell metabolic genes

Fig. 2

Unsupervised clustering identified 16 cell clusters, grouped into nine major cell populations(A). Marker gene expression was used to annotate cell types(B). T cells were classified into Tfh, cytotoxic T cells, double-negative T cells (DNT), naïve T cells, natural killer (NK) cells and Tregs(C). Representative marker genes for each T cell subtype are shown(D). Tregs were subdivided into two transcriptionally distinct subclusters(E). Pseudotime trajectory analysis revealed a dendritic differentiation structure and identified the starting point(F). Three states were observed along the trajectory: pre-branch and two terminal branches(G). Treg cluster C1 was mainly located at the end of branch 2; C2 was enriched at the end of branch 1(H). Dynamic expression of differentially expressed genes along pseudotime was visualized as heatmaps(I).

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