Extended Data Fig. 8: RIG-I depletion protected from kidney injury, cell death, and inflammation.
From: NAD+ precursor supplementation prevents mtRNA/RIG-I-dependent inflammation during kidney injury
a, Western blot quantification of RIG-I, MAVS, cleaved caspase-3 (cCASP3) in mice kidneys in indicated groups (n = 3 in each group). *P < 0.05, ***P < 0.001. NS, not significant. b, Experimental design. Renal tubule cells were isolated from WT and RIG-I KO mice. Relative transcript levels of Ifih1, cGAS, Isg15 and Irf7 in renal tubule cells of experimental groups (n = 3 in each group). Gene expression levels were normalized to Gapdh. *P < 0.05. NS, not significant. c, Kidney NAD+ levels in experimental groups (WT + PBS n = 4. RIG-I KO + PBS n = 4. WT + Cis n = 6. RIG-I KO + Cis n = 6). *P < 0.05. Data are presented as mean ± s.e.m. and were analyzed using a one-way ANOVA followed by Tukey post hoc test for multigroup comparison.
