Extended Data Fig. 6: Several ovarian features are not dramatically altered in Pnma1 and Pnma4 mutants.
From: The retrotransposon-derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity
(a-e) Ovaries from control (pooled wild type and heterozygote, gray), Pnma1−/− (cyan), Pnma4−/− (purple), or Pnma1−/− Pnma4−/− double mutant (red) mice were fixed, embedded, and PAS-stained. The following features were quantified per unit area (mm2) at the indicated times: (a) primary follicles, (b) secondary follicles, (c) corpora lutea, (d) post-ovulation follicles, and (e) atretic follicles. Statistical significance was determined by determined by one-way ANOVA with correction for multiple comparisons. Error bars indicate SEM. (f-i) GV oocytes were collected from ovaries of two-month-old wild-type and Pnma1−/− Pnma4−/− mice. We then injected mRNA encoding mClover-MAP4 (microtubule-binding protein) and H2B-mScarlet (histone) and imaged oocytes live for ~18 hours. (f) Representative images of the first meiotic division from the metaphase I (MI) plate (t = 0 min) to anaphase I (AI, t = 20 min). Misaligned chromosomes were counted at t = 0 min and lagging chromosomes at t = 12 min. (g) Time in hours (hr) between nuclear envelop breakdown to AI onset. (h) AI lagging chromosome quantification. (i) Misaligned chromosomes at on MI plate quantification (J) Misaligned chromosomes at on MII plate quantification (4 hours after meiosis I completion). (k, l) Continued data from Fig. 3. As in f-j, but GV oocytes were collected at 7 months. (k) Misaligned chromosomes at on MI plate quantification. (l) Misaligned chromosomes at on MII plate quantification (4 hours after meiosis I completion). Statistical significance was determined by determined by student’s t-test. Error bars indicate SEM (n.s. p > 0.05, * p ≤ 0.05).
