Extended Data Fig. 10: PNMA4 packages its own mRNA in a capsid-like particle.
From: The retrotransposon-derived capsid genes PNMA1 and PNMA4 maintain reproductive capacity
(a) Lysate was prepared from eight testes (collected at 3 months) from either wild type or control (lacking Pnma4) mice (3 biological replicates each). Lysate was fractionated by velocity (247,000 g, 3 hours) over a double sucrose cushion (25% and 70%). The 70% meniscus was further fractionated by isopycnic centrifugation on an iodixanol step gradient. PNMA4 was IPed from iodixanol fractions 8 and 9, RNA was extracted IP beads, and sequenced. Shown is a volcano plot of log2 fold change plotted against -log10 p-value of RNA-seq reads comparing wild type and controls. Values represent a ratio of enrichment between the wild type and control IPs. The further right the value, the more enriched the mRNA in the wild type IP. Genes with a log2 fold change greater than 1 and an enrichment p-value of less than 10e-4 are colored magenta. (b) Model for PNMA1 and PNMA4 function.
