Fig. 2: Comparison of in vivo and in vitro toxicity.

a, Comparison of effect concentrations for in vivo bioassays (EC10 for 10% growth inhibition in green algae (green, squares; Supplementary Table 9), EC10 for 10% immobilization in Daphnia magna (gold upward triangles; Supplementary Table 10), 50% effect concentration EC50 in the zebrafish (Danio rerio) behavioural assay for PMR (blue downward triangles; Supplementary Table 11)). There was not enough sample available to test W1 and W2 in D. magna or the zebrafish embryo, and testing was not possible for the filter samples in the algal toxicity assay, and the PMR in D. rerio was not significantly different from control up to REF 4 for the filtrate samples (unpaired t-test P = 0.6771). b, Comparison of effect concentrations in the in vitro neurotoxicity assay with EC10 for neurite outgrowth inhibition (10% reduction in neurite length, magenta diamonds) and inhibitory concentration IC10 for 10% cytotoxicity (teal circles) in the differentiated neuronal cell line SH-SY5Y (Supplementary Table 12).